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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by <t>ANCOVA</t> as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.
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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by <t>ANCOVA</t> as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.
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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by <t>ANCOVA</t> as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.
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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by <t>ANCOVA</t> as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.
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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by <t>ANCOVA</t> as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.
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Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by ANCOVA as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.

Journal: Nutrients

Article Title: The Role of Reduced Methionine in Mediating the Metabolic Responses to Protein Restriction Using Different Sources of Protein

doi: 10.3390/nu13082609

Figure Lengend Snippet: Assessment of impact of protein restriction on average food intake ( A ), average water intake ( B ), 96 h continuous energy expenditure in casein groups ( C ) and soy groups ( D ), average energy expenditure for all groups ( E ), Fgf21 mRNA and serum FGF21 ( F ), liver target genes ( G ), and inguinal white adipose tissue target genes ( H ). Food intake and water intake were measured at weekly intervals, and average food intake was expressed per unit body weight. The means of food intake and water intake were averaged over the entire study. Energy expenditure was measured continuously for 4 days after a 3-day equilibration period and calculated by ANCOVA as described in Materials and Methods. Serum and liver collected at end of study was used to measure FGF21 and target gene mRNA levels. Inguinal WAT collected at the same time was used to measure target gene mRNA levels. The respective end points were analyzed by one-way ANOVA and means annotated with different letters differ at p < 0.05. Data in each figure panel are presented as the mean ± SEM, n = 9–10.

Article Snippet: Group differences in 24 h EE (kJ/h/mouse) at study′s end were compared using Analysis of Covariance (ANCOVA) (JMP Software, Version 15; SAS Institute Inc., Cary, NC, USA) to calculate least squares means that accounted for variation in EE attributable to differences in lean mass, fat mass, food intake, and activity among the mice as before [ ].

Techniques: